MediaScout® Starter Kit

In order to start with separations on a 96 well format, Atoll offers a Starter Kit which enables you to develop a screening protocol on an 8-row, which can be then transferred to a full 96 plate.

At the same time this Starter Kit can be seen as the simplest chromatographic system in the world.

The Starter Kit consists of an 8-row of columns containing different media (listing below) stacked into a slider. This is sitting on a frame which is on top of a UV-readable 96-wellplate (e.g. UV-Star Greiner).

How to operate the Kit?
Using a positive liquid displacement pipette:
Set pipette into dispense mode. Regulate the speed to about 0.5 ml per minute
Please view the correlation between flowrate and selected Combitip Plus volumes
under "User Instructions(PDF, 86KB)"

Pipettes which work with our PipetColumns:
Brand HandyStep^®
Eppendorf Xstream^®
Gilson Repetman^®

• 1. Prepare the appropriate buffers.
• 2. Condition all columns with starting buffer
• 3. Apply sample(s)
• 4. Elute with appropriate buffer into adjacent wells by shifting the 8-row along the 12 positions
• 5. Read results in a plate reader

By this method 12 binding or respectively elution conditions can be checked.
To optimise buffer/solvent usage and preparation, please use for each chromatographic mode, a corresponding Kit.

MediaScout Starter Kit Q-Kit GE Capto Q GE Q-Sepharose 6 FF BioRad UNOsphere Q Merck Fractogel EMD TMAE Hicap M Merck Fractogel EMD TMAE MEDcap M Tosoh Toyopearl Super Q Pall-BioSepra Q Ceramic HyperD F AB Poros 50 HQ	MediaScout Starter Kit S-Kit GE Capto S GE S-Sepharose 6 FF BioRad UNOsphere S Merck Fractogel EMD SO3 M Merck Fractogel EMD SE Hicap M Tosoh Toyopearl MegaCap II SP 550 C Pall-BioSepra S Ceramic HyperD F AB Poros 50 HS
MediaScout Starter Kit HIC-Kit GE Phenyl SepharoseTM 6 FF high sub GE Phenyl SepharoseTM 6 FF low sub GE Butyl-S Sepharose 6 FF Tosoh Toyopearl Butyl 650 M Tosoh Toyopearl Super Butyl 550 C Tosoh Toyopearl Phenyl 650 M Merck Fractogel EMD Propyl S BioRad MacroPrep t-butyl	MediaScout Starter Kit IMAC Kit GE Chelating SepharoseTM FF GE IMAC SepharoseTM FF Merck Fractogel EMD Chelate M BioRad ProfinityTM IMAC Pall-BioSepra IMAC HyperCel® Tosoh Toyopearl® AF-Chelate-650M Sterogene Nickel Chelate Superflow Sterogene Cobalt Chelate Superflow
MediaScout Starter Kit Protein A Kit GE MabSelectTM GE MabSelect SuReTM GE MabSelect XtraTM GE Protein A SepharoseTM CL-4B Millipore PROSEP®-vA HC Millipore PROSEP®-vA Ultra Pall-BioSepra Protein A Ceramic HyperD® F AB Poros 50 A
MediaScout Starter Kit Standard HA BioRad Macro-Prep® ceramic hydroxyapatite Type I 40 µm BioRad Macro-Prep® ceramic hydroxyapatite Type II 40 µm BioRad Macro-Prep® ceramic hydroxyapatite Type I 80 µm BioRad Macro-Prep® ceramic hydroxyapatite Type II 80 µm BioRad Macro-Prep® ceramic fluoroapatite Type I 40 µm BioRad Macro-Prep® ceramic fluoroapatite Type II 40 µm BioRad Macro-Prep® ceramic hydroxyapatite Type I 20 µm BioRad Macro-Prep® ceramic hydroxyapatite Type II 20 µm

For further questions and inquiries call us or write to us at
Phone +49 (0) 751 - 56 121 0 from 8:30 a.m. to 5:00 p.m. (CET)
Fax      +49 (0) 751 - 56 121 70

Email  info@atoll-bio.com