MediaScout® PipetColumn Starter Kit
The world’s simplest and lowest cost chromatography system!

User instructions

In order to start with separations on a 96 well format, Atoll offers a Starter Kit, which enables you to develop a screening protocol on an 8-row, which can then be transferred to a full 96 plate.

At the same time this Starter Kit can be considered the simplest chromatographic system in the world.

The Starter Kit consists of an 8-row of columns containing different media stacked into a slider. This is sitting on a frame which is on top of a UV-readable 96-wellplate (e.g. UV-Star Greiner).

How to operate the Kit?
By using a positive liquid displacement pipette, e.g.:

• 1. Prepare the appropriate buffers.
• 2. Condition all columns with starting buffer
• 3. Apply sample(s)
• 4. Elute with appropriate buffer into adjacent wells by shifting the 8-row along the 12 positions
• 5. Read results in a plate reader

By this method 12 binding or respectively elution conditions can be checked in one experimental run.
In order to optimise buffer/solvent usage and preparation, please use a dedicated Kit for each chromatographic mode.

To ensure best sealing between pipette and column please press the pipette tip vertically and with ambient force into the column inlet!

For best performance please apply slow flow-rates (max. 0.5 ml/min = 150 cm/h)!

Pipette operation:

Brand Transferpettor/Gilson Microman:	Manually operated Flow-rate is set manually
Eppendorf Xstream:	Electrically operated Pick the lowest dispensing speed
Technical Data:	Bed height:10 mm Inner diameter:5 mm Column volume:200 µl • Materials, polypropylene (columns, adapters, filter 7-12 µm pores), POM (slider), alumina (adapter frame), silicone rubber (cap mats)

Eppendorf Multipette^® Xstream
Correlation between flow-rate and selected Combitip Plus volume

	Dispense - Speed 1		Dispense - Speed 2		Dispense - Speed 3
V Combitip Plus [ml]	Flow-rate [ml/min]	Linear flow-rate [cm/h]	Flow-rate [ml/min]	Linear flow-rate [cm/h]	Flow-rate [ml/min]	Linear flow-rate [cm/h]
0,1	0,17	50	0,25	75	0,35	100
0,2	0,34	100	0,50	150	0,70	200
0,5	0,86	250	1,25	375	1,75	500
1,0	1,70	500	2,50	750	3,50	1000
2,5	4,25	1250	6,25	1875	8,75	2500
5,0	8,60	2500	12,50	3750	17,50	5000
10,0	10,70	5000	25,00	7500	35,00	10000

  Dispense - Speed 1 is the lowest dispense - speed.

Equilibration

Sample application

Wash

Elution