MediaScout^® ResiQuot Technical Details and Function
The lower plate (3) of the device functions as a manifold chamber with vacuum connector and frit holder (11) with stainless steel frit.
The edge of the frit holder is completely sealed against the lower plate (3) by the gasket (9).
Vacuum aspiration can only take place through the stainless steel frit.
The precise alignment and orientation of the upper manifold (1) onto the lower manifold (3) is assured by two dowel pins placed near the edge of each unit and fitting into drilled holes.
Two knurled assembly screws (2) provide rapid and secure clamping of the upper and lower manifolds.
A gasket inserted into and around the edge of the upper manifold ensures a vacuum tight connection between upper and lower manifolds.
Once assembled, the perforated plate is inserted into the front opening as far as to the stop.
The 96 holes drilled in the perforated plate are then aligned to the centres of 96 larger holes in the upper manifold.
A vertical metal plate acts as a stop to ensure precise alignment and includes a gasket seal between the perforated plate and the assembled manifolds.
The perforated plate is held firmly in place by two clamps (4), attached to the front of the upper manifold.
The holes drilled in the upper manifold are filled with the required suspension which is then drawn into the 96 small cylindrical holes of the perforated plate under vacuum, to the level of the stainless steel frit.
As long as there is sufficient solid material in the suspension the cylindrical holes of the perforated plate are evenly and completely filled.
Excess solids remaining in the drilled holes of the upper manifold may be recovered after removal of the sample aliquots.
The perforated plate is released and withdrawn over wipers, which remove any residual solids from the plate surface. Compacted solid material is retained in the precisely filled cylindrical holes of the perforated plate.
The perforated plate is then placed above a 96 well collection microplate, and the compacted solids in the cylindrical holes are transferred into the wells using the 96 well format removal tool.
The pusher tool is precisely aligned to the 96 cylindrical holes by four guide pins located at the corners of the tool.
An example of resin aliquots obtained by transfer from the perforated plate into a microplate is shown in figure 3.Finally, excess solids are recovered from the uppermanifold.
The upper and lower manifolds are dissembled and the upper unit cleared of excess material using the 8-way pusher tool.
In this way, the unit may be used to sample a single suspension into 96 separate wells, or each well may be filled separately with materials of different composition depending on the needs of the user.


For further questions and inquiries call us or write to us at
Phone +49 (0) 751 - 56 121 0 from 8:30 a.m. to 5:00 p.m. (CET)
Fax      +49 (0) 751 - 56 121 70

Email  info(at)atoll-bio.com