Atoll
Information

Technical Information:

MediaScout® MiniChrom ValiChrom is a miniaturized low to medium pressure chromatography column, used in bioseparation applications, accommodating small sample volumes. All column parts are made from mechanically and chemically stable polymers, predominantly from polypropylene.

Column Type

Inner Diameter X Length

Column Cross Section

Column Volume

Flowrate1

Max. Flowrate1

 

(mm x mm)

cm2

ml

ml/min / cm/h

ml/min / cm/h

ValiChrom 5-X

5.0 x 20-300

0.2

0.4-15

0.5 / 150

< 5 / 1500

ValiChrom 8-X

8.0 x 20-600

0.5

1.0-30

0.5 / 150

< 5 / 1500

ValiChrom 11.33-X

11.3 x 20-600

1.0

2.0-60

2.5 / 150

< 25 / 1500

ValiChrom 16-X

16.0 x 20-600

2.0

4.0-120

5.0 / 150

< 25 / 1500

Connectors

standard HPLC (M10-32 UNF for 1/16 inch capillaries)

Maximum Operating Pressure1

30 bar

Chemical Stability2

Columns are tolerant to aqueous buffers and salt solutions, 1 M alkaline solutions, 1 M non-oxidizing mineral acids, 8 M urea, 6 M guanidine hydrochloride, non-halogenated organic solvents and detergents. They are not compatible with strong oxidants and halogenated solvents

1This depends on the chromatography material
2The chemical stability refers to the column hardware parts only. The individual packed chromatography media may have different stability. To avoid damage to the chromatography media, please refer to the relevant manufacturer’s data.
3The precise ID of the glass tube is 11.28 mm, corresponding to 1 cm2 cross section



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Used with chromatographic media of all types with particle size > 10µm

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Packing: Defined compression of chromatographic materials defined by

 

manufacturers specifications or customized.

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Storage medium: 20% Ethanol with or without 150mM NaCl, or customized

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Flowrate: Up to 10 ml/min

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Connections: Standard HPLC (M10-32 UNF for 1/16" tubing)

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Maximum working pressure: 50 bar

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Temperature ranges: During separation: -10°C - +60°C, storage: 10°C - 30°C

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Chemical Stability of column hardware:

 

pH 1-14, organic solvents (except halogenated), minerals and salts,

 

common eluents and additives (urea, antioxidants, thiols, detergents, tensides)

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Chromatographic performance: In accordance to separation material and the column geometry

 

(I.D. and bed height) and compression (e.g. 2,500 tp/m for Toyopearl 650 (M))

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Column Size: 5 mm I.D. x 10mm length , Volume 0.1 ml; fixed bed height

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Column Size: 5 mm I.D. x 25mm length , Volume 0.5 ml; fixed bed height

Column Size: 5 mm I.D. x 50mm length , Volume 1.0 ml; fixed bed height

Column Size: 8 mm I.D. x 20mm length , Volume 1.0 ml; fixed bed height

Column Size: 8 mm I.D. x 50mm length , Volume 2.5ml; fixed bed height

Column Size: 8 mm I.D. x 100mm length , Volume 5ml; fixed bed height

Column Size: 11.3 mm i.D. x 50mm length, Volume 5.0 ml; fixed bed height

Column Size: 11.3 mm i.D. x 100mm length, Volume 10 ml; fixed bed height