MediaScout® Starter Kit

In order to start with separations on a 96 well format, Atoll offers a Starter Kit which enables you to develop a screening protocol on an 8-row, which can be then transferred to a full 96 plate.

At the same time this Starter Kit can be seen as the simplest chromatographic system in the world.

The Starter Kit consists of an 8-row of columns containing different media (listing below) stacked into a slider. This is sitting on a frame which is on top of a UV-readable 96-wellplate (e.g. UV-Star Greiner).

How to operate the Kit?
Using a positive liquid displacement pipette:
Set pipette into dispense mode. Regulate the speed to about 0.5 ml per minute
Please view the correlation between flowrate and selected Combitip Plus volumes
under "User Instructions(PDF, 86KB)"

Pipettes which work with our PipetColumns:
Brand HandyStep^®
Eppendorf Xstream^®
Gilson Repetman^®

• 1. Prepare the appropriate buffers.
• 2. Condition all columns with starting buffer
• 3. Apply sample(s)
• 4. Elute with appropriate buffer into adjacent wells by shifting the 8-row along the 12 positions
• 5. Read results in a plate reader

By this method 12 binding or respectively elution conditions can be checked.
To optimise buffer/solvent usage and preparation, please use for each chromatographic mode, a corresponding Kit.

MediaScout Starter Kit Q-Kit GE Capto™ Q GE Q Sepharose™ FF BioRad UNOsphere™ Q Merck Fractogel® EMD TMAE Hicap (M) Tosoh Toyopearl® Q-600C AR Tosoh Toyopearl® GigaCap® Q-650M Pall-BioSepra Q HyperCel™ Applied Biosystems POROS® 50 HQ	MediaScout Starter Kit S-Kit GE Capto™ S GE SP Sepharose™ FF BioRad UNOsphere™ rapid S Merck Fractogel®EMD SE Hicap (M) Merck Eshmuno™ S Tosoh Toyopearl® GigaCap® S-650M Pall-BioSepra S HyperCel™ AB POROS® 50 HS
MediaScout Starter Kit HIC-Kit GE Phenyl Sepharose™ 6 FF LS GE Phenyl Sepharose™ 6 FF HS GE Butyl Sepharose™ 4 FF Tosoh Toyopearl® Butyl-650M Tosoh Toyopearl® Phenyl-600M Tosoh Toyopearl® PPG-600M Merck Fractogel® EMD Propyl (S) BioRad Macro-Prep® t-butyl	MediaScout Starter Kit IMAC Kit GE Chelating Sepharose™ FF GE IMAC Sepharose™ 6 FF Merck Fractogel® EMD Chelate (M) BioRad Profinity™ IMAC Pall-BioSepra IMAC HyperCel™ Tosoh Toyopearl® AF-Chelate-650M Sterogene Nickel Chelate Superflow Sterogene Cobalt Chelate Superflow
MediaScout Starter Kit Protein A Kit GE MabSelect™ GE MabSelect SuRe™ GE MabSelect Xtra™ Millipore PROSEP®Ultra Plus BioRad UNOsphere SUPrA™ Pall-BioSepra Protein A Ceramic HyperD® F AB Poros® 50 A POROS® MabCapture™ A
MediaScout Starter Kit Standard HA BioRad Macro-Prep® CHT™ Type I 40 µm BioRad Macro-Prep® CHT™ Type II 40 µm BioRad Macro-Prep® CHT™ Type I 80 µm BioRad Macro-Prep® CHT™ Type II 80 µm BioRad Macro-Prep® CFT™ Type I 40 µm BioRad Macro-Prep® CFT™ Type II 40 µm BioRad Macro-Prep® CHT™ Type I 20 µm BioRad Macro-Prep® CHT™ Type II 20 µm

For further questions and inquiries call us or write to us at
Phone +49 (0) 751 - 56 121 0 from 8:30 a.m. to 5:00 p.m. (CET)
Fax      +49 (0) 751 - 56 121 70

Email  info(at)atoll-bio.com