MediaScout® CentriColumn

High Throughput Sample Preparation

MediaScout® CentriColumn

Instruction Manual

MediaScout® CentriColumns are part of the 96 well MiniColumn platform from Atoll and are delivered customized, pre-packed with a broad range of commercially chromatography media. 

The available media are listed at Atoll’s web SHOP within a drop-down menu of the CentriColumn customizer.
 
CentriColumns are designed for high effective sample preparation as well as for downstream process development.
 
MediaScout® CentriColumns are operated by centrifugation and fit to any 96 DIN centrifuge able to accept the 41 mm high CentriColumn array excluding base collection plate.
 
MediaScout® CentriColumns provide:
  • Up to 384 samples at the same time 
 
 
 

CentriColumn characteristics

Plate size 128.3 x 86.0 x 14.0 mm
Plate material Polyoxymethylene copolymer
Number of wells 96
Column volume 50 µL          100 µL         200 µL    
Bed height 2.5 mm        5.0 mm        10.0 mm
Column inner diameter 5 mm
Column material Polypropylene
Chemical stability All commonly used aqueous buffers, pH 1 – 14, organic solvents1
Avoid Halogenated organic solvents, hexane
Storage solution
  • AIEX and CIEX: 20 % ethanol + 150 mM sodium chloride
  • HIC, SEC, AC and MMC: 20 % ethanol
  • CHT, CFT (Bio-Rad): 20 % ethanol + 10 mM sodium phosphate, pH 6.8
  • HyperCel™ (PALL): 30 % isopropanol + 0.1 M sodium phosphate, pH 4.3
  • ProSep® (Millipore): 1 % benzyl alcohol + 0.1 M sodium acetate, pH 5.1

Recommended storage temperature
  • AIEX, CIEX, HIC, SEC and MMC: +4°C to +30°C
  • AC: +4°C to +8°C
Working temperature +4°C to +30°C
Laser label

Placed on every individual column body and containing:

  • Product number
  • Resin name
  • Column volume in µL

 

1 Note: The chemical stability of the packed chromatography media has also to be taken into consideration. For more information please refer to the manufacture’s resin specification.